Salicylic acid increases flavonolignans accumulation in the fruits of hydroponically cultured Silybum marianum
(چکیده مقاله) :
Abstract :
Silybum marianum (L.) Gaertn. (Asteraceae) was hydroponically cultured using a nutrient film technique
system. Silibinin, isosilibinin and silychristin were detected in the fruits of the cultured plants. The effect
of salicylic acid on the improvement of flavonolignans production by the fruits of the hydroponically cultured
S. marianum was investigated. Salicylic acid was added to the nutrient solution at different concentrations
(100, 200 and 400 mM) and the mature fruits of the plant were collected five days after elicitor
addition. The fruits were then analyzed for their total flavonolignans contents and individual components
using quantitative proton nuclear magnetic resonance spectroscopy (qHNMR) and high-performance liquid
chromatography (HPLC). The results showed that elicitation with salicylic acid at 200 mM for five days
increased production of total flavonolignans (1.7-fold by qHNMR and 1.6-fold by HPLC) higher than the
control cultures and (1.4-fold by qHNMR and 1.1-fold by HPLC) higher than the cultivated plants.
Silychristin was the major flavonolignan produced by the cultured plant. Elicitation by 200 mM salicylic
acid increased silychristin production (1.6-fold by qHNMR and HPLC) higher than the control cultures
and (1.3-fold by qHNMR and 1.0-fold by HPLC) higher than the cultivated plants. The present study provides
a chance to improve secondary metabolite yield, serves as a useful tool for studying the biosynthesis
of these medicinally valuable compounds and its regulation in plant and spots more light on
hydroponic system as an important agricultural techn
system. Silibinin, isosilibinin and silychristin were detected in the fruits of the cultured plants. The effect
of salicylic acid on the improvement of flavonolignans production by the fruits of the hydroponically cultured
S. marianum was investigated. Salicylic acid was added to the nutrient solution at different concentrations
(100, 200 and 400 mM) and the mature fruits of the plant were collected five days after elicitor
addition. The fruits were then analyzed for their total flavonolignans contents and individual components
using quantitative proton nuclear magnetic resonance spectroscopy (qHNMR) and high-performance liquid
chromatography (HPLC). The results showed that elicitation with salicylic acid at 200 mM for five days
increased production of total flavonolignans (1.7-fold by qHNMR and 1.6-fold by HPLC) higher than the
control cultures and (1.4-fold by qHNMR and 1.1-fold by HPLC) higher than the cultivated plants.
Silychristin was the major flavonolignan produced by the cultured plant. Elicitation by 200 mM salicylic
acid increased silychristin production (1.6-fold by qHNMR and HPLC) higher than the control cultures
and (1.3-fold by qHNMR and 1.0-fold by HPLC) higher than the cultivated plants. The present study provides
a chance to improve secondary metabolite yield, serves as a useful tool for studying the biosynthesis
of these medicinally valuable compounds and its regulation in plant and spots more light on
hydroponic system as an important agricultural techn
(توضیحات تکمیلی) :
(توضیحات تکمیلی) :
Description :
مقاله ISI انگلیسی اصلی
سال انتشار: 2020
فایل ISI انگلیسی اصلی ، با فرمت Pdf
تعداد صفحات فایل ISI انگلیسی اصلی: 6 صفحه
سال انتشار: 2020
فایل ISI انگلیسی اصلی ، با فرمت Pdf
تعداد صفحات فایل ISI انگلیسی اصلی: 6 صفحه
Authors / Descriptions(نویسندگان/توضیحات): مقاله ISI سال انتشار2020 \ Hayam S. Ahmed, Abeer S. Moawad, Sameh F. AbouZid, Asmaa I. Owis
Sent date(تاریخ ارسال) :
1399/04/28 | 7/18/2020
Number of visits(تعداد بازدید):
581
Key words (کلمات کلیدی):
Silybum marianum , Hydroponic culture , qNMR , HPLC , Flavonolignans
Number of pages(تعداد صفحات) :
6
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