Nanomechanics and ultrastructure of the internal mammary artery adventitia in patients with low and high pulse wave velocity
(چکیده مقاله) :
Abstract :
The collagen-rich adventitia is the outermost arterial layer and plays an important biomechanical and
physiological role in normal vessel function. While there has been a lot of effort to understand the role
of the medial layer on arterial biomechanics, the adventitia has received less attention. In this study,
we hypothesized that different ultrastructural and nanomechanical properties would be exhibited in
the adventitia of the internal mammary artery (IMA) in patients with a low degree of arterial stiffening
as compared to those with a high degree of arterial stiffening. Human IMA biopsies were obtained from a
cohort of patients with arterial stiffening assessed via carotid-femoral PWV. Patients were grouped as low
PWV (8.5 ± 0.7 ms1, n = 8) and high PWV (13.4 ± 3.0 ms1, n = 9). Peakforce QNM atomic force microscopy
(AFM) was used to determine the nanomechanical and morphological properties of the IMA. The
nano-scale elastic modulus was found to correlate with PWV. We show for the first time that nanoscale
alterations in adventitial collagen fibrils in the IMA are evident in patients with high PWV, even
though the IMA is not involved in the carotid-femoral pathway. Our approach provides new insight into
systemic structure-property changes in the vasculature, and also provides a method of characterizing
small biopsy samples to predict the development of arterial stiffening.
physiological role in normal vessel function. While there has been a lot of effort to understand the role
of the medial layer on arterial biomechanics, the adventitia has received less attention. In this study,
we hypothesized that different ultrastructural and nanomechanical properties would be exhibited in
the adventitia of the internal mammary artery (IMA) in patients with a low degree of arterial stiffening
as compared to those with a high degree of arterial stiffening. Human IMA biopsies were obtained from a
cohort of patients with arterial stiffening assessed via carotid-femoral PWV. Patients were grouped as low
PWV (8.5 ± 0.7 ms1, n = 8) and high PWV (13.4 ± 3.0 ms1, n = 9). Peakforce QNM atomic force microscopy
(AFM) was used to determine the nanomechanical and morphological properties of the IMA. The
nano-scale elastic modulus was found to correlate with PWV. We show for the first time that nanoscale
alterations in adventitial collagen fibrils in the IMA are evident in patients with high PWV, even
though the IMA is not involved in the carotid-femoral pathway. Our approach provides new insight into
systemic structure-property changes in the vasculature, and also provides a method of characterizing
small biopsy samples to predict the development of arterial stiffening.
(توضیحات تکمیلی) :
(توضیحات تکمیلی) :
Description :
مقاله ISI انگلیسی اصلی
سال انتشار:2018
فایل ISI انگلیسی اصلی ، با فرمت Pdf
تعداد صفحات فایل ISI انگلیسی اصلی: 12 صفحه
سال انتشار:2018
فایل ISI انگلیسی اصلی ، با فرمت Pdf
تعداد صفحات فایل ISI انگلیسی اصلی: 12 صفحه
Authors / Descriptions(نویسندگان/توضیحات): مقاله ISI سال انتشار2018 \ Zhuo Chang a, Paolo Paoletti a, Steve D. Barrett b, Ya Hua Chim a, Eva Caamaño-Gutiérrez c, Maria Lyck Hansen d, Hans Christian Beck d, Lars Melholt Rasmussen d, Riaz Akhtar a,⇑
Sent date(تاریخ ارسال) :
1397/10/26 | 1/16/2019
Number of visits(تعداد بازدید):
756
Key words (کلمات کلیدی):
Arterial stiffening Artery Nanomechanics Collagen Atomic force microscopy PF-QNM
Number of pages(تعداد صفحات) :
12
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